Protocol for Reducing Cell Clumping in Single Cell ...
Slowly add 10 15 mL of medium or buffer containing 10% FBS dropwise, while gently swirling the tube. Rinse the vial with 1 mL of culture medium or buffer ( HBSS or PBS) containing 10% FBS to recover any remaining cells, and transfer the medium to the new tube. Top up the 50 mL tube with culture medium or buffer containing 10% FBS.
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