Kinetic studies with alkaline phosphatase in the presence and
· A very robust and inexpensive kinetic assay for determining rates of hydrolysis of pnitrophenyl phosphate by the enzyme alkaline phosphatase is reaction increases in rate with increase in pH. The enzyme is competitively inhibited by the reaction products, uncompetitively inhibited by Lphenylalanine, and responds to the presence of two .
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